Hi all!
Does anybody have experience with crystallization of proteins by slow
drifting of the pH? Let's say, for example, going from pH 7 to pH 5
over 1 week or 1 month. This is supposed to happen by diffusion of
volatile molecules like ammonia or acetic acid but I am having a hard
time finding any details or protocols.
I bet that there is a lot of anecdotal experience out there. Maybe
even references?
Thanks,
Jose.
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Jose Antonio Cuesta-Seijo
Cancer Genomics and Proteomics
Ontario Cancer Institute, UHN
MaRS TMDT Room 4-902M
101 College Street
M5G 1L7 Toronto, ON, Canada
Phone: (416)581-7544
Fax: (416)581-7562
email: [EMAIL PROTECTED]
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