Hi Eswar
Firstly, I would certainly try crystal seeding into random screens if
you haven't already tried it. Refs below.
Secondly, it's very convenient to grow the crystals under oil, and to
soak the organic solvents into the drops, through the oil. This makes
it much easier to harvest the
Dear All
I was working on a Human protein and expression and
solubility is good in E.coli and purification is One step (His-Tag), and i
need to cleave the Histag before screens, if not
the protein will precipitated and Aggregated, but after trying for 1.2 years
i have
Depending on how big your broccoli is I would simply crush them and mount as
big of a piece as possible and see how well it diffracts.
I assume you have tried already:
- glycerol
- changing protein:reservoir ratios
- temperature
- adding oil to either / and the reservoir, your drop
-crushed up
: [ccp4bb] Crystals with Organic solvents
Dear All
I was working on a Human protein and expression and
solubility is good in E.coli and purification is One step (His-Tag), and i
need to cleave the Histag before screens, if not the protein will precipitated
and Aggregated
