DNA carry-through in protein purification is quite common, especially if
you are working with basic proteins. A Talon step certainly doesn't
guarantee the removal of nucleic acid, and large fragments will
certainly run in the void volume of your gel-filtration column.
You can try washing the Ta
oard [mailto:[EMAIL PROTECTED] On Behalf Of Jacob
Keller
Sent: Tuesday, January 08, 2008 4:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] DNA contamination post-Talon?
Sorry for the off-topic question again,
but has anybody encountered DNA fragments' sticking to a Talon column? I got
th
