Pius,
Are you sure that you determined the correct cell. Which program did you
use? Usually there are much less spots on an image when a crystal has so
small unit size dimensions. To me the first crystal looks like protein.
Send it to a synchrotron and process the data in XDS. They can process
Hi Pius
DS_1.png -> protein diffraction !
DSA6E.png -> surface ice formation and protein crystal which did not survive
the freezing !!
DSA2F.png -> protein crystal which does not diffract, but cryo condition is
right !
Psp4f.png -> internal ice formation plus some surface ice also protein
crysta
Hi PS
What is the unit cell dimensions in the first crystal? It looks like
protein to me.
Maia
On 21/03/2011 2:03 PM, Pius Padayatti wrote:
Hi all,
We recently observed some diffraction from membrane protein crystallization
drops diffraction that look like non-proteinaceous (please see at
Working with the H+-ATPase AHA2 (C12E8/DDM/Cymal-5 detergent mixed
micelle) we always saw a ring at ~40Å which we attributed to the
micelle, but we have no experimental evidence that it was indeed caused
by detergents.
Heres a animated gif of this phenomenon:
http://www.bioxray.au.dk/~bjopp/pic
Hi All,
I am wondering if the detergent or lipid crystal can have diffraction at low
resolution.
If they can, what does the diffraction pattern looks like? Are there any
literatures describing these?
Many thanks!
