Hello,
I am fairly new to this lark so please forgive me if this question is unclear,
but it is really puzzling me.
I have used phaser to generate a molecular replacement structure of my target
(which has 100% identity to my template) and this particular crystal I had
soaked with a ligand.
I
Covalent modification?? Bias in 2fo-fc map?
Can you show us a pic of offending density?
Dave
On 28/12/2007, Brenda Patterson [EMAIL PROTECTED] wrote:
Hello,
I am fairly new to this lark so please forgive me if this question is
unclear,
but it is really puzzling me.
I have used phaser to
What's the resolution? At low res it is possible to miss a subtle movement
of e.g. some sidechains which are being replaced by the ligand. What quality
parameters did the MR have? Can you omit the entire ligand binding site,
refine, and re-generate the map - what does it look like after that?
A
There is another possibility that your ligand is not fully occupied,
if the binding site of protein endures an apparent change when bound
by ligand. Lijun
On Dec 28, 2007, at 7:55 AM, Brenda Patterson wrote:
Hello,
I am fairly new to this lark so please forgive me if this question
is
On Fri, 28 Dec 2007 15:55:39 +
Brenda Patterson [EMAIL PROTECTED] wrote:
Hello,
I am fairly new to this lark
No problem. With a name like Patterson, your future is guaranteed (unless of
course you see everything in the world with intensity but no phase).
I have a
