Dear Crystallographers,
Here is a summary of the methods for oligomeric stablization through
structure-informed engineered disulfide bonds (thanks to all who responded):
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SSBOND: Although the Google-accesible server seems to be down currently, it
can still be a
Hi Jacob,
Give SSBOND a try at http://129.128.24.248/forms/ssbond.html
Just upload your pdb and it will spit out pairs of residues that, if
mutated to cysteine, are able to form disulfides. For multi-domain
proteins I recommend to change residues numbers to 1-999 for chain A,
1001-1999 for ch
in my hands.
Good Luck,
Mike Thompson
- Original Message -
From: "Konstantin v. Korotkov"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, August 23, 2010 4:09:50 PM GMT -08:00 US/Canada Pacific
Subject: Re: [ccp4bb] Disulfide Designer Program
Hello,
You could try Disulfide by
Hello,
You could try Disulfide by Design:
http://cptweb.cpt.wayne.edu/DbD/
Good luck,
Konstantin
On Mon, 23 Aug 2010, Jacob Keller wrote:
Dear Crystallographers,
I remember having heard of a program which takes a given oligomeric assembly,
and suggests optimum disulfides to stablize the com
Dear Crystallographers,
I remember having heard of a program which takes a given oligomeric
assembly, and suggests optimum disulfides to stablize the complex. Can
someone refresh my memory which program that is, and where it is available?
Best Regards,
Jacob Keller
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