[ccp4bb] Dye for protein affinity measurement

2012-02-08 Thread Jiang Jiahong
Dear all, I am looking for some kind of dye for protein affinity comparison, but do not know which to choose. I know protein A can contact B to form a complex,now I hope to find something simiar with A to act as an inhibitor to block the process of A-B complex formation. Maybe a short peptide

Re: [ccp4bb] Dye for protein affinity measurement

2012-02-08 Thread Patrick Shaw Stewart
Jiahong Thermo sells a series of kits called DyLight Fluor for fluorescent labelling of antibodies or other proteins. They have everything you need and they're very convenient and easy to use. You can pick the excitation and emission wavelength. If you label both A and B (or C) with different "

Re: [ccp4bb] Dye for protein affinity measurement

2012-02-08 Thread Xiaodi Yu
binding depending on your purpose. And another option (a little "dangerous" ), is using radio active to label one of your protein. Yu Xiaodi Date: Wed, 8 Feb 2012 14:17:47 + From: patr...@douglas.co.uk Subject: Re: [ccp4bb] Dye for protein affinity measurement To: CCP4BB@JISCMAIL.AC.U

Re: [ccp4bb] Dye for protein affinity measurement

2012-02-08 Thread Vandana Kukshal
(a little "dangerous" ), is > using radio active to label one of your protein. > > Yu Xiaodi > > -- > Date: Wed, 8 Feb 2012 14:17:47 +0000 > From: patr...@douglas.co.uk > Subject: Re: [ccp4bb] Dye for protein affinity measurement >