People who would know (and know whether such a strain would even be viable)
are Gary Cecchini at UC San Francisco/VA and Bob Gennis at Univ of Illinois
at Urbana. In case you are not already talking with them.
It has been reported that an assembly factor (SDH5 or SDHE or SDHAF2)
is required for flavination of both proteins, without which you would have
no activity. So knocking out that one gene may be sufficient. It has
been reported to be a little leaky however, perhaps more so at
higher temperature. Meaning that SDH5-independent flavination has
been seen in some cases. And fumarate reductase has activity with
non-covalently bound FAD, but only for fumarate reduction not succinate
oxidation, so if "any kind of Complex II activity" only includes the
forward reaction, you would be OK.
There's a group in New Zealand working n SDHE in bacteria- pubmed SDHE
and see what is their latest, and they may have the knockout (of SDHE).
eab.
On 04/04/2017 10:54 AM, Fulvio Saccoccia, Sapienza wrote:
Dear ccp4ers,
I was wondering if someone does know (and hence could let me know in
turn) an E.coli strain which lacks both of the sdh (succinate
dehydrogenase) and frd (fumarate reductase) operon, so that bacteria do
no longer retain any activity from complex II or similar. I know the
question is off-topic but I am confident about your kindness.
Cheers
Fulvio
--
Fulvio Saccoccia, PhD
Institute of Cell Biology and Neurobiology (IBCN)
Consiglio Nazionale delle Ricerche
Via E. Ramarini, 32 - 00015 Monterotondo Scalo Roma (RM)
tel.+390690091244
