Co expression of binding partner perhaps ? And then tagging the other protein
partner ?
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Dear Wenhe,
this sounds a bit like our struggles to solubly express and purify the
dsRNA-binding avian reovirus sigmaA protein, please find the two papers here:
http://www.ncbi.nlm.nih.gov/pubmed/?term=van+Raaij+MJ%2C+sigmaA
The key to solving our problem was to realise the protein aggregated esp
Dear CCP4 friends,
I have been searching around the CCP4 mails for the discussion of soluble
protein aggregations, as I have the same problem recently. Many efforts I
have tried but without success. Here, I would like to summarise what I have
done and maybe any of you come across some ideas.
1. M
