A little off from the original question. Why don't small crystals dissolve to
make a bigger crystal, especially when the small ones grow on top of each
other? Can the clustered 3D crystals (I think it is called macroscopic twin) be
used for full data collection?
Again, thank you.
Theresa
Bosch, Juergen wrote:
Hi Dirk,
I remember a neat paper don't recall who wrote it. I think it was in Acta D
where the
authors made a tiny probe the size of an elongated crystal glued to a
[/Advertisement on]
Hampton loop [/Advertisement off]. The probe was a temperature sensor and they
recorde
> Sad was the day when I mounted this puppy and it shot to 8-10A. Room
> temperature. And messing around with cryos didn't help either.
But if that puppy had been smaller, it might have diffracted even
worse, and just think if that little icy crystal had been bigger...
JPK
>
>
> Can't remember
I'm going with Jurgen on this one.
http://img27.imageshack.us/img27/3232/pastedgraphic1.png
Sad was the day when I mounted this puppy and it shot to 8-10A. Room
temperature. And messing around with cryos didn't help either.
Can't remember the size, but I think I had scooped it with a 0.8 mm
Jürgen Quote: "Propane for whatever reason has gone extinct in certain
areas of the world :-) ."
I went to SSRL (Stanford) with a colleague who wanted to use liquid
propane. We had to go through a mound of paper work to get permission
bring propane on site and set up the experiments. I do
: Tuesday, February 07, 2012 11:52 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Freezing crystal
BIGGER is not always BETTER?
Theoretically it should be better because you have more scattering
matter.
Enrico: what you state above is only true if you ignore absorption of
the X-ray by the
On 02/07/12 11:12, Dirk Kostrewa wrote:
Dear Jürgen,
Am 07.02.12 16:58, schrieb Bosch, Juergen:
Then one last remark, LN2 versus cryo-stream freeze. Dipping in LN2
leads to a quicker freeze of your material.
Are you sure? There was a publication by Warkentin et al. [1] about a
cold gas lay
Just a thought for those that mentioned propane and ethane, I would like to
suggest that they try carbon tetrafluoride (CF4) instead. It certainly should
be much safer. It melts at 90 K and boils at 145 K, so you know you are below
145 K if you see it as a liquid.
Hi Dirk,
I remember a neat paper don't recall who wrote it. I think it was in Acta D
where the authors made a tiny probe the size of an elongated crystal glued to a
[/Advertisement on] Hampton loop [/Advertisement off]. The probe was a
temperature sensor and they recorded the cooling rate under
Dear Jürgen,
Am 07.02.12 16:58, schrieb Bosch, Juergen:
Then one last remark, LN2 versus cryo-stream freeze. Dipping in LN2
leads to a quicker freeze of your material.
Are you sure? There was a publication by Warkentin et al. [1] about a
cold gas layer above liquid nitrogen that reduces the
ginal Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Theresa H. Hsu
Sent: Monday, February 06, 2012 11:00 PM
To:
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK><mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Freezing crystal
Hi all
Thanks f
resa H. Hsu
Sent: Monday, February 06, 2012 11:00 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Freezing crystal
Hi all
Thanks for all the suggestions which I will try soon.
How do the crystallization condition (PEG vs. salts like ammonium
sulfate
board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Theresa H. Hsu
Sent: Monday, February 06, 2012 11:00 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Freezing crystal
Hi all
Thanks for all the suggestions which I will try soon.
How do the crystallization condi
; From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
>> Theresa H. Hsu
>> Sent: Monday, February 06, 2012 11:00 PM
>> To: CCP4BB@JISCMAIL.AC.UK
>> Subject: Re: [ccp4bb] Freezing crystal
>>
>> Hi all
>>
>> Thanks for all the suggestions which I wi
Behalf Of
> Theresa H. Hsu
> Sent: Monday, February 06, 2012 11:00 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Freezing crystal
>
> Hi all
>
> Thanks for all the suggestions which I will try soon.
>
> How do the crystallization condition (PEG vs. sa
H. Hsu
Sent: Monday, February 06, 2012 11:00 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Freezing crystal
Hi all
Thanks for all the suggestions which I will try soon.
How do the crystallization condition (PEG vs. salts like ammonium
sulfate) affect the croyprotectant condition? Do factors
Hi all
Thanks for all the suggestions which I will try soon.
How do the crystallization condition (PEG vs. salts like ammonium sulfate)
affect the croyprotectant condition? Do factors like presence of low
concentration of high molecular weight PEG (> 2000) mean PEG is better? Do
buffers and sa
CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Theresa
H. Hsu
Sent: Sunday, February 05, 2012 5:49 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Freezing crystal
Hi all
Is there a list of conditions to be tried *first* for cryoprotectant? My
crystals diffract at room temperature capi
Theresa,
Several suggestions that have been given are excellent advice:
Li salts suggested by Tommi Kajander is what I would use
in particular:
80% saturated lithium sulfate.
This should work. I would be very surprised if it does not.
Malonate as suggested by Sean Seaver is another great idea,
Biochemistry
University of Houston
From: Tommi Kajander
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, February 6, 2012 1:04:13 AM
Subject: Re: [ccp4bb] Freezing crystal
if you use oil do direct dry Paratone-N, with paraffin oil is not as good.
Li-salts should
work also - i
On Sun, 2012-02-05 at 22:49 +, Theresa H. Hsu wrote:
> Crystals are from 2 M ammonium sulfate.
Sodium malonate is your friend
http://scripts.iucr.org/cgi-bin/paper?fw5004
--
Oh, suddenly throwing a giraffe into a volcano to make water is crazy?
Dear Theresa
Cryo-cooling the crystals straight out of their drops or after brief
incubations in crystal stabilization solutions containing >2M ammonium sulfate
may be the way to go.
Here is a copy/paste piece from Kyndt et al (2007) Biochemistry 46, 95-105.
All the best
Savvas
Aliquots (0.5 í L
if your crystals are from 2 M AmSO4 without buffer, try to measure the pH in
the drop, if possible.
Or if you have plenty of crystals, transfer to 2 M AmSO4 buffered at a wide
range of different pHs to see where the crystals are stable, before adding
cryoprotectant.
In the end, you may need to g
if you use oil do direct dry Paratone-N, with paraffin oil is not as good.
Li-salts should
work also - i would almos imgaine you can freeze directly from so high
(NH4)2SO4 conc.
but perhaps not. little bit (10%) glycerol probably does it also..
Tommi
On Feb 6, 2012, at 12:55 AM, Vineet Gaur wr
Hi Theresa,
A good place to start when searching for suitable cryo conditions are the
tables in these references:
Garman, et al. J. Appl. Cryst. (1996). 29, 584-587.
McFerrin, et al. J. Appl. Cryst. (2002). 35, 538-545.
hope they help and good luck.
Eric
__
Eric Larson
Boehrin
Hi Theresa,
Try glycerol at 10 % with 5 % increments up to 30 % v/v. You may also be able
to attain protection by increasing the ammonium sulfate concentration along
with incremental glycerol approach.
Crystallization of RNA/protein complexes by Garber and colleagues (Acta Cryst.
(2002). D58,
Hi Theresa,
Once I had crystals in 3.5 M Amm. Sulfate. I used Paraffin oil and
Paraton-N-oil (in 1:1 ratio). I also used 30% Xylatol.
Best,
Vineet
On Sun, Feb 5, 2012 at 5:49 PM, Theresa H. Hsu wrote:
> Hi all
>
> Is there a list of conditions to be tried *first* for cryoprotectant? My
> crysta
Hi all
Is there a list of conditions to be tried *first* for cryoprotectant? My
crystals diffract at room temperature capillary but no in 30% PEG 400. Crystals
are from 2 M ammonium sulfate.
Thank you.
Theresa
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