Hi Veronica,
as others have said, at RT the radicals travel much further than at cryo
temperature, so your lateral shifts are probably not sufficient. But it is not
clear to me that radiation damage is to blame at all. To me it sounds like you
might have to mask your beamstop better, or you are
Dear Gerard, Eleanor et al.,
In the graphs of Rmeas vs. batch number (and I or I/sigma vs. batch
number), everything looks roughly constant, which made me think that
significant radiation damage was not the issue--but are you saying that
radiation damage might also (or instead) produce a smiley in
Dear Veronica,
At first glance, it looks as if you have a textbook example of
progressive radiation damage, i.e. the structure is changing between
the start and the end of your experiment, and the scaling gets skewed
towards finding a best compromise between all the measurements at
medium (ra
That is a fantastically low Rmerge ! What are the actual numbers?
You might expectslightly higher Rmerge for stronger reflections? i.e. at
low resin..
Eleanor
On 27 February 2015 at 20:40, Veronica Pillar wrote:
> Hi all,
>
> I have a data set from a large room-temperature lysozyme crystal
> con
Hi all,
I have a data set from a large room-temperature lysozyme crystal consisting
of 6 90-degree sweeps, each taken from a fresh spot on the crystal. When I
scale & merge the first sweep by itself, the R[meas/merge] vs. resolution
trace as reported by aimless looks fairly normal (lowest values i