An excellent review of this subject was published not long ago:
http://dx.doi.org/10.1107/S0907444905032130
It is even open access!
But, in general the "trick" to improving diffraction is to get your
molecules clean, get them to all adopt the same conformation, and then
sit still. Adding a
got a ligand? Stick in in there!
> Do you have an expression tag? Cleave it/leave it on.
> What is in your protein buffer? Mess about with that?
> Temperature gradients
> How is your purity?
> How quickly are you getting this in to trays? Can you speed this up?
> Might be a
: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How can improve diffraction quality
Dear ccp4 user
I am facing one crucial problem regarding diffraction. Actually the size of my
crystal is good enough 0.5mm but it was diffracted only 4 A.
The conditions of crystallization recipes are 4.5% PEG 1000
ncipal Beamline Scientist
Diamond Light Source
Harwell Science and Innovation Campus
Chilton
OX11 0DE
UK
Tel. 01235 778057
Mob. 07920 138148
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Afshan
Begum
Sent: 18 October 2011 12:45
To: ccp4bb
Subject: [ccp4bb] How can improve di
Dear ccp4 user
I am facing one crucial problem regarding diffraction. Actually the size of my
crystal is good enough 0.5mm but it was diffracted only 4 A.
The conditions of crystallization recipes are 4.5% PEG 1000, 0.1M Tris and 25mM
Na citrate. I really need your suggestions regarding ho
