Dear Peng,
The choice of asymmetric unit is somewhat arbitrary. Please forgive me and
ignore this if I am saying something obvious and you already know this does not
apply in your case, but I see most of your previous posts concern EM
structures, so perhaps you are relatively new to solving
Dear Nicolas,
Thank you for your help.
Our DNA is not a palindromic sequence, and I think it should not be degraded
easily.
The data can be processed in the space groups C2221, P41212 or lower, with
12bp, 6bp ,24bp or longer in one NCS. I can see the connection between two
symmetric DNA
Dear Peng,
to me your problem sound a bit strange, except if it's a palindromic
sequence. I don't understand how you can have one part of the DNA in one
asymmetric unit and one in another one. My question are : maybe you
considering a NCS as a true symmetry and underestimate the unit-cell
Hello, everyone，
Recently, we solve a protein-DNA complex. 20bp-DNA was used for
crystallization, but only 6bp was found in one symmetric unit.
My question is：
How to define the DNA bond between P and O3’ from two different symmetric units
during my refinement?
Peng
