Re: [ccp4bb] How to get rid of Membrane formed on hanging droplets?

2007-03-07 Thread Maneesh Yadav
lf of David Briggs Sent: Mon 3/5/2007 7:46 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] How to get rid of Membrane formed on hanging droplets? Hi, What you describe is "skin" - rumoured to be denatured protein. http://xray.bmc.uu.se/~terese/crystallization/pics/SKIN2.JPE<http://xray.b

Re: [ccp4bb] How to get rid of Membrane formed on hanging droplets?

2007-03-07 Thread Frank von Delft
heers Frank > -Original Message- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On > Behalf Of Edwin Pozharski > Sent: 07 March 2007 15:55 > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] How to get rid of Membrane formed on > hanging droplets? > >

Re: [ccp4bb] How to get rid of Membrane formed on hanging droplets?

2007-03-07 Thread Edwin Pozharski
I had some luck once cutting the crystal out of skin with a razorblade. When the skin is extremely sticky, you tend to get your crystal wrapped up in it (which may be OK - you just get more background scatter) and carefully cutting skin on all four sides may be a better option. Wang, Yeming (NIH/N

Re: [ccp4bb] How to get rid of Membrane formed on hanging droplets?

2007-03-05 Thread Kris Tesh
of Membrane formed on hanging droplets? Hi, What you describe is "skin" - rumoured to be denatured protein. http://xray.bmc.uu.se/~terese/crystallization/pics/SKIN2.JPE <http://xray.bmc.uu.se/%7Eterese/crystallization/pics/SKIN2.JPE> And it's a pain, especially when your

[ccp4bb] How to get rid of Membrane formed on hanging droplets?

2007-03-05 Thread David Briggs
Hi, What you describe is "skin" - rumoured to be denatured protein. http://xray.bmc.uu.se/~terese/crystallization/pics/SKIN2.JPE And it's a pain, especially when your crystals stick to it. Best suggestions are: 1) Try screening ad

[ccp4bb] How to get rid of Membrane formed on hanging droplets?

2007-03-05 Thread Wang, Yeming (NIH/NIEHS) [F]
Dear everyone, I am working on crystallization of a protein/RNA complex recently. The crystals were initially grown from BICINE(9.0) 0.1M, 1,4-Dioxane 2%(v/v) , PEG 20,000 10%(w/v), at 10mg/ml. I noticed that there was a membrane formed on the surface of the hanging droplets. This membrane seem