The plates I used were from Hampton, 72 well microbatch plate, untreated
hydrophobic ones, HR3-087.
I thought about other plates once. But what I had in mind was the thing
matters would be the oil since the skin appeared at the interface.
So I tried different oils for the plates, including
This happened to me previously, and the only thing that worked for me was to
lower the protein concentration. I was setting my protein up at 10 mg/mL,
only to find skins on nearly all the drops. I would damage the crystals
trying to get them out of the drops... I lowered my protein concentration
Lower the concentration did help a little bit. I didn't the 6 mg/ml setups,
but still got some skins.
You are right the skins are more likely to find in the condition of
PEGs...but also find in other conditions.
Lower the conc. of PEGs is a good advice, and I'll try it. Thanks.
Shu
On Sun, Sep 6,
Hi, all.
I'm doing the crystallization screening for my protein.
In over 1/2 of my hanging drops, there were skins formed on the surface of the
drop.
So I switched to use microbatch, and again almost 4/5 drops under the oil were
skins at the interface.
I tried to add additives, like detergents,
