Date: Sat, 22 May 2010 11:17:41 +0800
From: rh_ibp2...@hotmail.com
Subject: [ccp4bb]
To: CCP4BB@JISCMAIL.AC.UK
Dear all,
Recently, I am working on a complex which includes two protein subunits. The
interaction was based on the Zinc Finger motif of one protein. I co-purified
the complex
Dear Heng,
Tris forms only a very weak complex with Zn2+. It is unlikely that the
problem arises from Tris. It might be simply that the complex
dissociates in the presence of high imidazol when you elute the complex
from the nickel column. Imidazol is a pretty good Zn2+ coordinating
molecule. DTT i
