Tiancen,
While not entirely impossible - this is a formidable task. The answer to your quesiton depends on the specifics of your situation and on what additional information you are able to procure. Quite a few kinases have specific sites; equal or greater numbers are only partially specific to a greater or lesser extent. For instance, unregulated PKA will phosphorylate as many Ser and Thr residues as it can find, provided that steric hindrance isn't an issue. This is of course somewhat of an extreme example. What other information is available to you? Artem _____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of TC Hu Sent: Monday, October 27, 2008 4:17 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Is it possible to predict the kinase when knowing a phosphorylated substrate sequence? Dear all, Sorry for the non-CCP4 question. Could anyone please tell me is there a way to predict the kinase when knowing a phosphorylated substrate sequence? Thanks in advance! Cheers Tiancen Hu Ph.D. Shanghai Institute of Materia Medica Chinese Academy of Sciences
