Dear CCP4bb,
I'm refining an antibody structure which requires Kabat residue numbering with
insertion codes. My setup of Refmac5 and Buster both break peptide bonds
between some (not all) of the residues with insertion codes. I was wondering
whether there is a special way of handling these
/divdivSubject: [ccp4bb]
Kabat, insertion codes refinement /divdiv
/divDear CCP4bb,
I’m refining an antibody structure which requires Kabat residue numbering with
insertion codes. My setup of Refmac5 and Buster both break peptide bonds
between some (not all) of the residues with insertion codes. I
Hi David -
Your input files for Refmac (I’m not sure about Buster) should have LINKR
records of the form:
LINKRGLY L 95 THR L 95A gap
This has worked fine for me in the past. The file I happened to excerpt here
was refined with Refmac 5.6.0117
for
the final model if needed for comparative analysis.
Ed
Sent on a Sprint Samsung Galaxy S® III
Original message
From: Hargreaves, David
Date:06/16/2014 6:07 AM (GMT-05:00)
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Kabat, insertion codes refinement
Dear
