Hi,
I need some advice from experts. I have a low resolution electron density
map (3.7 angstroms) solved by SIRAS. The protein is a tetramer (47
kDa/monomer) but I'm having trouble getting in register. There is very good
main chain density and clear secondary structure (beta-sheets) so I likel
nu Das,
JCSG,
SSRL-SLAC.
From: CCP4 bulletin board on behalf of Alexei Datsuk
Sent: Fri 6/15/2007 10:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Low resolution chain tracing
Hi,
I need some advice from experts. I have a low resolution electron density map
Hi,
Ah yes, low resolution chain tracing I'm only in the initial
stages of map interpretation, but I anticipate I might have a similar
problem. I would love to hear from anyone who has ideas as well.
Thanks
Jeff
On Jun 15, 2007, at 10:03 AM, Alexei Datsuk wrote:
Hi,
I need some
Hi,
you might be able to distinguish the disulfide bonds from normal
backbone when looking at the log-likelihood residual maps in SHARP:
even for data collected at about 1A we often see anomalous peaks for
sulfurs (and the data doesn't even have to be very good). And a S-S
should be fairly strong.
