Are thw Se SAd and native data isomorphous - because if so you can just
cad the data sets together - generate phases for the Se set, then use
those as a starting set for the native data to phase extend. Parrot is
the more up to date version of DM which does this in the GUI.
The AutoSHARP will
th that, you might be able to combine phases from
this procedure with the Selenium SAD phases, as already suggested.
Kianoush
--- On Thu, 9/1/11, Basudeb Bhattacharyya
mailto:bbhattach...@wisc.edu>> wrote:
From: Basudeb Bhattacharyya
mailto:bbhattach...@wisc.edu>>
Subject:
combine phases from
this procedure with the Selenium SAD phases, as already suggested.
Kianoush
--- On Thu, 9/1/11, Basudeb Bhattacharyya wrote:
From: Basudeb Bhattacharyya
Subject: [ccp4bb] Low resolution structure determination advice
To: CCP4BB@JISCMAIL.AC.UK
Date: Thursday, September 1
How about phase extension using DM, sure you say you only have one mol per asu
but it might still be worth trying various approaches of solvent
flattening/flipping.
Don't know what you used to detect your sites and refine them, but it also
might be worth sticking them into Sharp with your partia
Hi,
Depending on how many zn sites you have, you may be able to do zn-mad
for your native crystals. You don't mention if you've tried combining
your various sources of phase information; if not, it's worth looking into.
You may also want to look into various multi-crystal techniques
(averagi
Dear all,
We're looking for some advice about how to proceed with a structure we're
working on. Our protein is 750 amino acids and naturally binds zinc. We have
a SeMet data set that goes down to 3.7 angstroms. 4 of 8 selenium sites are
ordered and visible in addition to our zincs and we've
