Thank you very much for your comments.
I re-do the data process with P21, and use the output for Phaser.
This time, Phaser finishes with good resolution, the TFZ is 28. And the
model is tetramer.
Cheers
Uma
On Thu, Aug 2, 2012 at 9:45 PM, Roger Rowlett wrote:
> A few thoughts:
>
>1. Sea
C222 means 2 possible space groups C222 and C2221.
P222 means 8 possible space groups.
ps: I don't understand the C2221A and C222A listed by phaser when I chose
the "alternative space groups (listed)" option in C222 space group, because
when I chose "all alternative space group", only C222 and C22
A few thoughts:
1. Search for all possible space groups (e.g., P2 and P21 in this
case). Be happy it isn't C222, which means 8 possible combinations
of screw axes to search! As mentioned already, P21 is far more
common than P2. I think P21 is one of the most common space groups
in pro
On Wed, Aug 1, 2012 at 11:27 AM, Uma Ratu wrote:
> The protein is in tetramer form. I define this by using the residue number
> (1332) which is 4 x monomer.
>
> After run, Phaser only gave 9 partial solutions, and no solution with all
> components. The resulted PDB contains only dimer form of the
Dear All:
I try to use Phaser to solve the structure by Molecular Replacement.
The data set is collected @180 degree. I process the data using HKL, and
have resonable good score: rejection (0.05), Linear R-factor (0.038),
completeness (98.3), resolution (50-1.5).
I then use Phaser to do MR. The
