Did you grow Malic Acid crystals?
I see no reason why glycerol cannot be the precipitant. I am aware that
at least one protein is crystallized with ethylene glycol as the
precipitant.
On Wed, 9 Jan 2008, JINJIN ZHANG wrote:
Dear All,
I have got large and nice crystals in a condition that
t: [ccp4bb] Malic Acid interferes heavy metal binding?
Dear All,
I have got large and nice crystals in a condition that contains 40%
glycerol, 75 mM DL-Malic Acid pH7.0, and that's it! No precipitant?! No
buffer?! I'm so comfused. As far as from the product website, the Malic A
Dear Jinjin:
Malic acid, a diprotic acid, has a pKa of about 3.4 for the first dissociation
and a second at about 5.1. So it is best as a buffer within a pH range of about
2.4 to 6.1, and it at its best around best in the range of 3.4 to 5.1. If the
pH of your solution is indeed 7, then it is
Dear All,
I have got large and nice crystals in a condition that contains 40% glycerol, 75 mM DL-Malic Acid pH7.0, and that's it! No precipitant?! No buffer?! I'm so comfused. As far as from the product website, the Malic Acid has no buffering function. I don't know whether it's a precipitant. The
