Re: [ccp4bb] Membrane Protein Crystallization Plates

2012-02-27 Thread Patrick Shaw Stewart
Yuri I know this isn't quite what you're asking, but it's helpful to use the COC (UV permeable) version of plates if you're crystallizing samples that contain detergent. It's just that the drops tend to spread very thinly if you use the normal polystyrene PS plates. The COC is more hydrophobic.

Re: [ccp4bb] Membrane Protein Crystallization Plates

2012-02-27 Thread Pius Padayatti
Hi i do not want to get into trouble by going against any products The plates what you are talking about as soon it came out we tested. I did not look back to see if there was a monoolein:cholestrol coated plate. The ones i used were for sure monoolein coated ones. That tells it all.The rest of

Re: [ccp4bb] Membrane Protein Crystallization Plates

2012-02-27 Thread Jim Fairman
Yuri, Did you mean plates for setting up Lipidic Mesophases? If so, here is a listing of products I have used in the past. I highly endorse the plates from Molecular dimensions, particularly the plastic laminex plates (MD11-51-100 + MD11-54). They do have the dis-advantage of drying out after

Re: [ccp4bb] Membrane Protein Crystallization Plates

2012-02-27 Thread Pius Padayatti
QuoteParticulalrly the monoolein and monoolein/cholesterol coated plates ( I am not sure I can mention the vendor here but it should not matter) Since the person who asked this question here forget about it alltogether to write something back here is what he was asking about (i think) Anybody

Re: [ccp4bb] Membrane Protein Crystallization Plates

2012-02-27 Thread Yuri Pompeu
Thanks for all the replies. I will try a couple of different plates/set-ups. My favorite will be the one that gives me a crystal ;)

[ccp4bb] Fwd: [ccp4bb] Membrane Protein Crystallization Plates

2012-02-26 Thread Pius Padayatti
Padayatti Sent: 26 February 2012 01:08 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Membrane Protein Crystallization Plates Hi Yuri, i strongly suggest these plates sold through Hampton research Paul Marienfeld GmbH plates for your use. here is a link , best plates in the market. link: http

[ccp4bb] Membrane Protein Crystallization Plates

2012-02-25 Thread Yuri Pompeu
Hello Everyone, I am considering the purchase of crystallization plates for membrane proteins. I would love to hear what some of the community thinks or has experienced with these. Particulalrly the monoolein and monoolein/cholesterol coated plates ( I am not sure I can mention the vendor here

Re: [ccp4bb] Membrane Protein Crystallization Plates

2012-02-25 Thread Pius Padayatti
Hi Yuri, i strongly suggest these plates sold through Hampton research Paul Marienfeld GmbH plates for your use. here is a link , best plates in the market. link: http://hamptonresearch.com/product_detail.aspx?cid=10sid=182pid=611 Plates comes with extra cover slips in addition to single glass