Hi.
We are trying to prepare heavy atom derivatives of a protein in which
a surface residue was mutated to cysteine. Mass spectrometry of the
purified cysteine mutant showed an additional peak with a molecular
mass that correspond to a mercaptoethanol-bound cysteine mutant. I am
Hi Bill:
Thanks for the prompt reply. I will try purifying the protein without
mercaptoethanol.
Wataru
On 2009/05/13, at 11:42, William G. Scott wrote:
HI Wataru:
I think it makes an R-S-S-R' bond, so the disulfide will prevent the
heavy atom from binding, unless exchange is rapid.
