We recently published a method for estimating F(000) by simply equating
it to Fcalc(000), the sum of all the electrons in the refined model
(including those in the bulk solvent):
http://dx.doi.org/10.1073/pnas.1302823110
The results of this procedure compared favorably with the two
experimen
Starting with the density (or specific volume) of water, you can obtain
rho(water)=0.33 e-/A^3. It's a nifty little bit of arithmetic and dimensional
analysis.
-MW
Michael C. Wiener, Ph.D.
Professor
Department of Molecular Physiology
and Biological Physics
University of Virginia
PO Box 800886
Dear All,
Is there a way or program that is good at providing a solid estimate of F000
using the standard mean protein electron density of 0.433 e/A**3 and a mean
solvent electron density calculated from the crystallization conditions? I am
wondering how the latter might affect F000 if the con
