You dont say how close your sequence ID is for your new protein and the
model. It is often very hard to kick start refinement with low homology
And what about internal symmetry
Is there a Non crystallographic translation?
Does the self rotation function show a relationship between the two
mole
CCP4 bulletin board wrote on 05/10/2007 09:31:59
PM:
> Hello,
> I'm an undergraduate and recently crystallized and obtained 2.9A
> diffraction data for a protein which is predicted to fold into a WD40
> 7-bladed beta-propeller structure (which has been crudely verified by
> cryo-EM by another lab
Hello Scott,
hmmm - it is quite difficult to do a good analysis of your problem,
remotely. You've tried the enantiomorphic space group I4(3), just to be
sure? In principle, the molecular replacement solution given by Phaser
sounds good, but this is no proof of whether it's correct. What sounds
Hello,
I'm an undergraduate and recently crystallized and obtained 2.9A
diffraction data for a protein which is predicted to fold into a WD40
7-bladed beta-propeller structure (which has been crudely verified by
cryo-EM by another lab). The space group appears to be I4(1) with
unit cell pa
