Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the
condition to improve the size of the crystal and reduce the number of
nucleation.
The crystals are coming from five to six conditions , all have PEG 3350 in
common.
Apart from PEG, t
: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Multiple nucleation
Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the
condition to improve the size of the crystal and reduce the number of
nucleation.
The crystals are coming from five to six
half Of
Jobichen Chacko
Sent: 30 March 2007 00:45
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Multiple nucleation
Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the
condition to improve the size of the crystal and reduce the number of
You said you tried macroseeding, but what about microseeding? It works
quite well in many cases. Just don't forget that you should find a zone
were nucleation does not happen before you seed. Take a look at this paper:
Bergfors, T. (2003) Seeds to Crystals, J. Struct. Biol., 142, 66-76.
There
, March 29, 2007 7:45 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Multiple nucleation
Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the
condition to improve the size of the crystal and reduce the number of
nucleation.
The crystals
Not a contradiction at all :) If the protein supply is adequate - this is
a perfectly good approach to explore. I simply forgot to mention it.
I've also not mentioned engineering the protein's surface (works really
well for us because we have secret ways to do this, learned from ancient
and myster
I've found that crystallization in sitting drops under oil dramatically reduces
the no. of nucleation events and increases the overall crystal size. Now, if
the increased crystal size helps improve diffraction is something to be tested.
Among the many other suggestions...
Raji
-Includ
