On Tue, Apr 13, 2010 at 09:24:13AM -0400, Nathaniel Clark wrote:
> [...] If you don't want to buy those tools just try 2 nylon loops, and lots
> of patience.
Or use cats whiskers which you can fix into a Pasteur pipette with wax, nail
varnish, plasticine, etc.
I find them to have just the right s
MiTeGen has a set of soft plastic microtools that are inteded for this
kind of stuff, and I have had suscess using them to break out single
crystals from clumps or fused crystals. You can either load in a
pencil or mount in a base.Also nylon loops. You might need to
have one tool in each hand
Amit Sharma,
I second Tim's suggestions.
The first experiment is the following.
Set up several experiments at constantly decreasing precipitant
concentrations.
Streak seed all of them. If in any of those you can get non-clustered
crystals
non matter what size, seeding can solve your problem
Have you tried anything, yet? The list of answers can be quite long.
A couple of keywords:
- micro-seeding
- macro-seeding
- cross-seeding
- additive screens
- extend to purification protocol
- change temperature, pH, etc.
- have you search around the current conditions
...
if you describe a litt
One way to separate crystal cluster is to poke
them with a tiny probe, e.g. Hampton Research HR-217. Then scoop up a
single crystal or large fragment for screening/data collection.
Cheers.
On 4/12/2010 3:31 PM, Amit Sharma wrote:
Dear all,
I am getting protein crystals in clusters. How c
Dear all,
I am getting protein crystals in clusters. How can I achieve isolated
crystals.
Thanks
Amit Sharma
