Re: [ccp4bb] Off-topic (SPR)

2013-05-08 Thread Zhijie Li
@JISCMAIL.AC.UK Subject: [ccp4bb] Off-topic (SPR) Dear pals, Sorry for my off-topic question but its been a while I am challenging to get a 15 kDa protein coated on CM5 chip. Calculated PI of protein is 5.9 & I tried Acetate buffer pH5.2 and 3.8 for coating under normal procedure

[ccp4bb] Off-topic (SPR)

2013-05-08 Thread Jahan Alikhajeh
Dear pals, Sorry for my off-topic question but its been a while I am challenging to get a 15 kDa protein coated on CM5 chip. Calculated PI of protein is 5.9 & I tried Acetate buffer pH5.2 and 3.8 for coating under normal procedure of Biacore. After immobilization I saw this message: response lev