With regard to low levels of expression: Is there a particular reason
to use 293 cells? Transient expression in Cos-1/Cos-7 cells gives
~ 10 fold higher levels of expression because of the plasmid amplification
in these cells. (The only requirement is that the plasmid should
have SV40 ori; most ma
Dear Dima,
thank you for your reply
Since this is ectodomain, I presume it contains native sorting sequence.
The ectodomain contains a secretion signal: either its native or
another secretion signal. I have tested a few other, strong secretion
signals to boost the expression levels with m
Kenneth,
> sorry for this off topic question. I am producing the ectodomain of a
>
> human receptor in HEK293T cells by transient expression. The
> expression
> levels are quite low. The protein contains a high number of disulfide
> bridges. It has been reported that overexpression of protei
Dear all,
sorry for this off topic question. I am producing the ectodomain of a
human receptor in HEK293T cells by transient expression. The expression
levels are quite low. The protein contains a high number of disulfide
bridges. It has been reported that overexpression of protein disulfide
