12:12:28 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Optimization from needle shaped crystals
>
> I can share what has worked for my crystals :
>
> 1. You can put a grid across your condition with same or altered drop
> ratios.
>
> 2. You can try micro seedi
Dear prem,
I am sharing my experience with needle crystal that worked for me.
1.First check that salt or a protein crystal by SDS PAGE or mass
spectroscopy.
2. For improvement of morphology and size, you could try microseeding of
your crushed crystal, generally needle crystal appear quickly, fur
/david_briggs
From: CCP4 bulletin board on behalf of chitra latka
Sent: Sunday, September 8, 2019 12:12:28 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Optimization from needle shaped crystals
I can share what has worked for my crystals :
1. You can put
I can share what has worked for my crystals :
1. You can put a grid across your condition with same or altered drop
ratios.
2. You can try micro seeding. (This has given me the best results so far).
3. You can try Hampton's additive screen.
On Sun, Sep 8, 2019 at 10:09 AM Prem Prakash wrote:
Dear Prem,
before moving on with any optimization rounds it would be good to first confirm
that these crystals are actually protein crystals, and in particular the
protein of interest.
For example, a Silver-stained SDS-PAGE gel can be very informative.
You can run a couple of your crystalline c
optimized condition.
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From: CCP4 bulletin board on behalf of Prem Prakash
Sent: Sunday, September 8, 2019 10:09:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Optimization from needle shaped crystals
De
