Re: [ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli?

2009-01-20 Thread Anthony Addlagatta
d first. > > Artem > > -Original Message- > From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of > Torres-Larios Alfredo > Sent: Monday, January 19, 2009 8:26 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Posttranslational modifications of r

Re: [ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli?

2009-01-19 Thread Artem Evdokimov
ail.ac.uk] On Behalf Of Torres-Larios Alfredo Sent: Monday, January 19, 2009 8:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli? Dear all, I know this sounds weird (and besides, it's a non CCP4 question), but does a

[ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli?

2009-01-19 Thread Torres-Larios Alfredo
Dear all, I know this sounds weird (and besides, it's a non CCP4 question), but does anyone know any reports or has worked with a recombinant protein that is apparently post translationally modified by E. coli? We have an enzyme with two populations that have been sequenced at the N-terminus