Ding ding ding ding...we have a winner:
A search model with 30% identity was submitted to the Phyre
server which returned a target sequence-corrected model
The Phyre monomer was "dimerized" in Pymol using symmetry
operators from a previous MR solution us
Hi Roger,
I think your ideas are sound, but I would add some "prime-and-switch"
density modification in resolve plus/minus ncs to try and improve the maps
and cut down on phase bias.
Hth,
Dave
--
Hand delivered by Androids
On 1 Sep 2010 16:12, "Roger Rowlett" wrote:
I am trying to find a MR
Hi Roger,
personally, I would take the four dimers from Phaser, strip off all side
chains (make a poly-ala/gly), do a ML refinement with tight NCS
restraints. The resulting map could then be on-the-fly
real-space-averaged with Coot. At your resolution, I would expect to see
the real register
I am trying to find a MR solution for a large unit
cell (R3:H, 158x158x196) with a relatively poor, but I think workable
search model (30% identity, 50% similarity). The data set is decent to
2.4 A. I might be able to get better if necessary. I submitted the
sequence of the target to the Phyre
