; [duskyde...@gmail.com<mailto:duskyde...@gmail.com>]
> Envoyé : jeudi 24 juillet 2014 13:50
> À : CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
> Objet : [ccp4bb] Problem in molecular replacement
>
> DEAR ALL
> I am trying to solve the structure of a protein DNA complex
JISCMAIL.AC.UK] de la part de dusky dew [
duskyde...@gmail.com]
> Envoyé : jeudi 24 juillet 2014 13:50
> À : CCP4BB@JISCMAIL.AC.UK
> Objet : [ccp4bb] Problem in molecular replacement
>
> DEAR ALL
> I am trying to solve the structure of a protein DNA complex with
molecular replacement. T
s has over-hang.
Nicolas
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de dusky dew
[duskyde...@gmail.com]
Envoyé : jeudi 24 juillet 2014 13:50
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Problem in molecular replacement
DEAR ALL
I am trying to solv
In addition to that good suggestion, with a model at such high sequence
identity and with reasonable resolution data, it would be a good idea to search
for smaller models, like a single dimer or even a monomer, because the assembly
may have changed conformation. Another possibility you should c
Try using DNA as a search model - this has worked very successfully in our
hands before.
Tony.
- - - - - - - - - - - - - - - - - -
Dr Antony W Oliver
Senior Research Fellow
CR-UK DNA Repair Enzymes Group
Genome Damage and Stability Centre
Science Park Road
University of Sussex
Falmer, Brighton,
DEAR ALL
I am trying to solve the structure of a protein DNA complex with molecular
replacement. The resolution in about 2.5 angstrom. The spacegroup is P21.
The search model has about 50% identity and is a dimer of a dimer. The
problem is the unit cell is huge and so the number of molecules in as
