Influenza polymerase heterotrimer expressed in insect cells (MultiBac
technology) as a fully synthetic polyprotein with subunits separated by
TEV sites
TevProtease-Tev-PA-Tev-PB1-Tev-PB2-Tev-CFP
Reich et al. Nature 2014; doi:10.1038/nature14009
/Extended Data Figure 1 | Production and char
Dear colleagues
We have been working on a protein that is produced as a pro-peptide, cleaved
internally and reassembled into a complex. The interacting regions are the new
C and N-termini at the cleavage site, so no rearrangement or loss of part of
the protein is involved. The resulting interac
