From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Minyun Zhou
[minyunz...@gmail.com]
Sent: Wednesday, July 30, 2014 9:58 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Pseudo-translation problem
Dear all,
I am trying to determine the structure of a protein-DNA complex. I
Hi,
Lattice-translocation disorder is one possibility.
How does the diffraction image look like?
Are there any streaks?
Best regards,
Takanori Nakane
On 2014-07-30 15:58, Minyun Zhou wrote:
Dear all,
I am trying to determine the structure of a protein-DNA complex. I
collected several
Dear all,
I have an orthorhombic crystal (pointless suggests most likely space groups P 2 21 21 or P 2 21 2)
with two molecules expected in the asymmetric unit. Analyzing the native Patterson map I found the
following peaks (in fractional coordinates):
CELL 63.0400 117.2500 133.6500
The general rule is that a Patterson peak should be ~ 20% of the origin
before considering it significant so none of those would really need to be
considered.
Eleanor
On 25 February 2013 12:48, Michele Lunelli efu...@yahoo.it wrote:
Dear all,
I have an orthorhombic crystal (pointless
Hi all,here is a question from a beginner. I have a home source data set
that indexed and scaled in a P2 space group (a=46.704,b=59.362, c=48.783,
alpha=90.0, beta=104.469, gamma=90.0) with predicted 2mol/au. After failing
to get a MR solution with Phaser I ran the phenix.xtriage which showed
Dear colleagues,
I would like to thank J. Murray, J. Wright, K. Futterer, E. Dodson, A. Forster,
and F. Long for responding to my posting of two days ago on pseudo-translation
vectors in molrep vs other programs (see original posting at the end of this
message).
I should have said at the outset
Dear colleagues,
For a particular MR problem I am dealing with, 'analyse_mr' suggests
that there maybe a pseudo-translation vector as evidenced by the very
significant non-origin peaks in the native patterson: e.g
GRID 80 112 80
CELL 104.8290 151.2840 109.4910 90. 118.1310 90.
I think the error is in BALBES - there is a peak I guess at 0.95 0 0.01
but it must be too close to the origin to be a translation vector from
one molecule to another.
There are reasons for such peaks - sometimes spurious large terms in the
data..
but they dont usually represent true
Hi,
I suggest you check which version of MOLREP you used. Currently,
BALBES now actually use MOLREP in 'auto' mode for PST. The two should
be the same. The difference may be because BALBES uses the latest
version of MOLREP.
Fei
On 7/30/07, Eleanor Dodson [EMAIL PROTECTED] wrote:
I think the
