Hello,
I wanted to know if there is a standard procedure for purification of
protein with ligand. I have never done this before so it will be nice to
get some help.
Thanks,
Mariah
--
Mariah Jones
Department of Biochemistry
University of Florida
Hi Mariah,
You may need to specify what type of ligand (is it a nucleotide, a
small synthetic molecule, a peptide etc ?) and also what is the affinity
between your ligand and your protein.
I have purified several protein-ligand complexes, you can go several routes.
If you have a high affinit
ckens no good
intentions are in his mind.
_
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
protein.chemist protein.chemist
Sent: Thursday, February 26, 2009 5:58 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Purification with ligand
Hello,
I wanted to know i
