You may as well have fun by manually building your molecule B into 1.9A ed map
when R-factors are already in mid 30s.
Vaheh
From: CCP4 bulletin board On Behalf Of Sam Tang
Sent: Monday, November 6, 2023 2:02 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] RES: [ccp4bb] About model building
Dear all
Thanks again for more input to the question. And credits to Eleanor and Kay
for pointing out the high R-factor and possible issue with the space group.
Their advice prompted me to revisit the MR solution and it happens that
another solution, in P3121, gave a better map with R-factor after
Hi Sam.
If you still have any of your crystals or any protein solution left in the well
you harvested your crystals, I would run a MS/MS with them. Next step would be
to run AF with your known chain A and your best Mass Spec hit (s), and use the
resulting model for MR.
Good luck
Rafael Marqu
