Dear users, I'm just approaching to a new project...so, I have evidences of an interaction between the N-terminus region of a protein with its own C-terminal domain. The C-terminal domain is perfectly folded (easily expressed and purified), instead of the N-terminal one that is mostly random coil. I would like to express and crystallize the complex between them. I'm wondering if anyone could give me tricks how to express the random coils portion in E. coli and keeping it in a soluble form. Could co-expression help?
Thank you in advance, all the best, Marco
