Re: [ccp4bb] Re :Re: [ccp4bb] MAD phasing

2009-05-26 Thread Eleanor Dodson
You need to try both P61 22 and P6522 with the Se sites changed fron x,y,z to -x,-y,-z. The anom differences can be explained by either solution. But maybe these programs automatically check both hands? Eleanor Dodson Ethayathulla Abdulsamath wrote: hi BertThank you for your reply. Your are

[ccp4bb] Re :Re: [ccp4bb] MAD phasing

2009-05-21 Thread Ethayathulla Abdulsamath
hi BertThank you for your reply. Your are right about the values for your Pattersons and NatFourier, but the systematic absences and pointless suggested me to be P6122 and according to mathews coeff i get nbsp; 1 molecule with Vm=nbsp; 2.06 solvent=nbsp; 40.42. My protein has two SeMet sites so

[ccp4bb] Re :Re: [ccp4bb] MAD phasing

2009-05-21 Thread Ethayathulla Abdulsamath
hello peteri tried phenix.autosol. It gave me following results.nbsp; but the data analyses says that my data pseudo translational symmetry, I have pasted it. Do phenix handle pseudotranslation. Need suggestion on thisSolution # 1nbsp; BAYES-CC: 15.3 +/- 27.3 Dataset #1nbsp;nbsp; SG: P 61 2