gt; ---------------------
>
>
> TWINNING SUMMARY
>
>
> Twinning fraction from H-test: 0.42
>
> Twinning fraction from L-Test: 0.18
>
>
> It is highly probable that your crystal is TWINN
his (if not, then not
>> a “good reviewer.”)
>>
>>
>>
>> JPK
>>
>>
>>
>> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
>> *Alex Lee
>> *Sent:* Friday, April 14, 2017 11:50 AM
>>
>> *To:* CCP4BB@
not a “good
reviewer.”)
JPK
From: CCP4 bulletin board
[mailto:CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>] On Behalf Of Alex
Lee
Sent: Friday, April 14, 2017 11:50 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Refmac5 twin refinement pushing
eviewer will have you do this (if not, then not
>> a “good reviewer.”)
>>
>>
>>
>> JPK
>>
>>
>>
>> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
>> *Alex Lee
>> *Sent:* Friday, April 14, 2017 11:50 AM
>
017 11:50 AM
>
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Refmac5 twin refinement pushing Rfree
> surprisingly down
>
>
>
> Thanks Eleanor, I tried MR for P32 21 and P32 12.
>
> SG P3221: SOLU SET RFZ=5.3 TFZ=8.8 PAK=0 LLG=121 TFZ==11.2 LLG=944
> TFZ==29.2 P
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Refmac5 twin refinement pushing Rfree surprisingly down
Thanks Eleanor, I tried MR for P32 21 and P32 12.
SG P3221: SOLU SET RFZ=5.3 TFZ=8.8 PAK=0 LLG=121 TFZ==11.2 LLG=944 TFZ==29.2
PAK=0 LLG=944 TFZ==29.2
SOLU SPAC P 32 2 1
SG P3212:
Solution #1 annotation
just try with the
>> higher-symmetry point group first, see what happens.
>>
>>
>>
>> JPK
>>
>>
>>
>> *From:* Alex Lee [mailto:alexlee198...@gmail.com]
>> *Sent:* Thursday, April 13, 2017 11:32 PM
>>
>> *To:* Keller, Jacob
>> *Cc:*
;
> *To:* Keller, Jacob
> *Cc:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Refmac5 twin refinement pushing Rfree
> surprisingly down
>
>
>
> Hi Keller,
>
>
>
> Thanks for the suggestions! I only have two copies in ASU at SG P32.
> Zanuda also suggests P32 i
-symmetry point group first, see what happens.
JPK
From: Alex Lee [mailto:alexlee198...@gmail.com]
Sent: Thursday, April 13, 2017 11:32 PM
To: Keller, Jacob
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Refmac5 twin refinement pushing Rfree surprisingly down
Hi Keller,
Thanks for the suggestions
hese types of
> things!
>
>
>
> JPK
>
>
>
> *From:* Alex Lee [mailto:alexlee198...@gmail.com]
> *Sent:* Thursday, April 13, 2017 9:08 PM
> *To:* Keller, Jacob
> *Cc:* CCP4BB@JISCMAIL.AC.UK
>
> *Subject:* Re: [ccp4bb] Refmac5 twin refinement pushing Rfree
&g
works out—I am interested in these types of things!
JPK
From: Alex Lee [mailto:alexlee198...@gmail.com]
Sent: Thursday, April 13, 2017 9:08 PM
To: Keller, Jacob
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Refmac5 twin refinement pushing Rfree surprisingly down
Hi Keller,
I do not how to check
://doi.org/10.1107/S2059798316019318
>
>
>
> Jacob
>
>
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
> *Eleanor
> Dodson
> *Sent:* Thursday, April 13, 2017 3:11 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Refmac5 twin refinem
f Of Eleanor
Dodson
Sent: Thursday, April 13, 2017 3:11 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Refmac5 twin refinement pushing Rfree surprisingly down
Twin refinement cannot be compared directly to untwinned - the R factors are
between different parameters - without twinning it is assumed
3 april 2017 19:19
> *Aan: *CCP4BB@JISCMAIL.AC.UK
> *Onderwerp: *[ccp4bb] Refmac5 twin refinement pushing Rfree surprisingly
> down
>
>
> Dear All,
>
> I have a protein/dna complex crystal and data collected at 3A and another
> set at 2.8A, space group P32. L test sh
fraction.
Cheers,
Robbie
Sent from my Windows 10 phone
Van: Alex Lee<mailto:alexlee198...@gmail.com>
Verzonden: donderdag 13 april 2017 19:19
Aan: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Onderwerp: [ccp4bb] Refmac5 twin refinement pushing Rfree surprisingly down
Dear Al
Dear All,
I have a protein/dna complex crystal and data collected at 3A and another
set at 2.8A, space group P32. L test shows twinning (fraction around 0.11).
The structure solved by MR and model building of the complex finish (no
solvent built yet, I do not think it's good to build solvent in su
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