Jun Yong and Jobichen
(I've mentioned this before, but ) - both of your projects jump out as very
good targets for microseeding with random screens. This method often gives
extra hits and better crystals because it is more likely that crystals will
grow in the metastable zone. It often reduces
Hey Jun,
If it's an old batch check and see if you have microorganisms living in the
stock or proliferating in the drop - see the paper by Bai et al:
doi:10.1107/S1744309107002904
In this paper they demonstrated how they could not reproduce a crystal hit
from an old screen up until they realized a
I once had a similar problem. The crystals only reproduced when grown in
condition containing PEG 8000 from SIGMA but not from FLUKA. This difference
may not affect crystals from other proteins but some proteins are more
sensitive to slight changes than others. Since you found out that the
Hi all,
Recently, I produced crystals with MBClass1-64 which contains PEG4000, HEPES-Na
and NaCl. But, I struggled to reproduce crystals. I tried to set up tray with
different batch of solution. I got the crystals only from 2008 solution, but
not from fresh ones. I asked technical service of
Maybe your old solution evaporated then you end up in your old tube with a
more concentrated solution in PEG and NaCl so try to screen with new
conditions with higher PEG and/or NaCl concentration
Mick
2011/4/12 Jun Yong Ha j...@princeton.edu
Hi all,
Recently, I produced crystals with
or PEG 4000 got old. ask around in the department or university for old PEG
4000 bottles.
good luck!
Berta
On Apr 12, 2011, at 3:28 PM, mickael blaise wrote:
Maybe your old solution evaporated then you end up in your old tube with a
more concentrated solution in PEG and NaCl so try to
.
--
From: Jun Yong Ha j...@princeton.edu
Sent: Tuesday, April 12, 2011 7:56 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Reproducing crystals.
Hi all,
Recently, I produced crystals with MBClass1-64 which contains PEG4000,
HEPES-Na and NaCl. But, I struggled to reproduce crystals. I
PS, it might be a good time to start an additive screen.
--
From: Jun Yong Ha j...@princeton.edu
Sent: Tuesday, April 12, 2011 7:56 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Reproducing crystals.
Hi all,
Recently, I produced crystals
deg C and cool them
down, then use them. Let us know what happens.
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jun
Yong Ha
Sent: Tuesday, April 12, 2011 6:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Reproducing crystals.
Hi all,
Recently
.
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jun
Yong Ha
Sent: Tuesday, April 12, 2011 6:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Reproducing crystals.
Hi all,
Recently, I produced crystals with MBClass1-64 which contains PEG4000,
HEPES
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