Re: [ccp4bb] Reproducing crystals.

2011-04-14 Thread Patrick Shaw Stewart
Jun Yong and Jobichen (I've mentioned this before, but ) - both of your projects jump out as very good targets for microseeding with random screens. This method often gives extra hits and better crystals because it is more likely that crystals will grow in the metastable zone. It often reduces

Re: [ccp4bb] Reproducing crystals.

2011-04-14 Thread Chen Guttman
Hey Jun, If it's an old batch check and see if you have microorganisms living in the stock or proliferating in the drop - see the paper by Bai et al: doi:10.1107/S1744309107002904 In this paper they demonstrated how they could not reproduce a crystal hit from an old screen up until they realized a

Re: [ccp4bb] Reproducing crystals.

2011-04-14 Thread Raj Gosavi
I once had a similar problem. The crystals only reproduced when grown in condition containing PEG 8000 from SIGMA but not from FLUKA. This difference may not affect crystals from other proteins but some proteins are more sensitive to slight changes than others. Since you found out that the

[ccp4bb] Reproducing crystals.

2011-04-12 Thread Jun Yong Ha
Hi all, Recently, I produced crystals with MBClass1-64 which contains PEG4000, HEPES-Na and NaCl. But, I struggled to reproduce crystals. I tried to set up tray with different batch of solution. I got the crystals only from 2008 solution, but not from fresh ones. I asked technical service of

Re: [ccp4bb] Reproducing crystals.

2011-04-12 Thread mickael blaise
Maybe your old solution evaporated then you end up in your old tube with a more concentrated solution in PEG and NaCl so try to screen with new conditions with higher PEG and/or NaCl concentration Mick 2011/4/12 Jun Yong Ha j...@princeton.edu Hi all, Recently, I produced crystals with

Re: [ccp4bb] Reproducing crystals.

2011-04-12 Thread Berta Martins
or PEG 4000 got old. ask around in the department or university for old PEG 4000 bottles. good luck! Berta On Apr 12, 2011, at 3:28 PM, mickael blaise wrote: Maybe your old solution evaporated then you end up in your old tube with a more concentrated solution in PEG and NaCl so try to

Re: [ccp4bb] Reproducing crystals.

2011-04-12 Thread Zhijie Li
. -- From: Jun Yong Ha j...@princeton.edu Sent: Tuesday, April 12, 2011 7:56 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Reproducing crystals. Hi all, Recently, I produced crystals with MBClass1-64 which contains PEG4000, HEPES-Na and NaCl. But, I struggled to reproduce crystals. I

Re: [ccp4bb] Reproducing crystals.

2011-04-12 Thread Zhijie Li
PS, it might be a good time to start an additive screen. -- From: Jun Yong Ha j...@princeton.edu Sent: Tuesday, April 12, 2011 7:56 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Reproducing crystals. Hi all, Recently, I produced crystals

Re: [ccp4bb] Reproducing crystals.

2011-04-12 Thread Jim Pflugrath
deg C and cool them down, then use them. Let us know what happens. -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jun Yong Ha Sent: Tuesday, April 12, 2011 6:57 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Reproducing crystals. Hi all, Recently

Re: [ccp4bb] Reproducing crystals.

2011-04-12 Thread Kendall Nettles
. -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jun Yong Ha Sent: Tuesday, April 12, 2011 6:57 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Reproducing crystals. Hi all, Recently, I produced crystals with MBClass1-64 which contains PEG4000, HEPES