Hello, Two positions available in my lab at EMBL Grenoble: 1. Post doc position via the BioStruct-X Joint Research Activity (JRA) workpackage "Mammalian cell toolbox for structural biology" (collab. with Oxford University). 2. Research technician position.
Details pasted below. Further info and online application at www.embl.org/jobs. Thanks, Darren Research technician, High-Throughput Protein Expression Location: Grenoble, France Staff Category: Staff Member Contract Duration: 1 year Grading: 4 or 5, depending on experience and qualifications Closing Date: 31 October 2012 Reference number: GR_00044 Job Description The European Molecular Biology Laboratory (EMBL) is one of the highest ranked scientific research organisations in the world. The Headquarters Laboratory is located in Heidelberg (Germany) and the outstations are in Grenoble (France), Hamburg (Germany), Hinxton (UK) and Monterotondo (Italy). A technician position is available in the High Throughput Protein Technologies laboratory of Darren Hart at EMBL Grenoble. It is funded by P-CUBE and BioStruct-X, European “Research Infrastructures” projects established to provide access to unique high-throughput protein production facilities by visiting international researchers. The candidate will be responsible for molecular biology and automation tasks associated with high throughput screening of expression constructs in bacteria using a new robotic technology, ESPRIT. Projects will be conducted with both local and international visitors, and the candidate will be responsible for the planning of the work and visits, together with experimental supervision. In addition to the project support roles, the candidate will work on a set of defined research activities involving vector design, protocol development and crystallisation studies. Tasks include DNA purification and enzymatic manipulation, PCR, operation of robotics and other advanced instruments, protein expression and purification. Additional tasks include the development of new protocols, testing of new equipment for the facility, keeping accurate records of consumable use on a project basis, ordering of laboratory consumables and monitoring associated budgets, meeting with visiting representatives from suppliers, general maintenance of the robotic laboratory equipment and arranging repairs as necessary. Qualifications and Experience The candidate should hold a Masters degree, or have a good level of previous, relevant work experience. He/she should have a sound experimental background in DNA manipulation methods, E. coli protein expression and protein purification. Experience in high throughput expression, protein engineering, directed evolution and/or laboratory automation would be advantageous.The candidate should have experience of working in an English speaking environment.The ability to work with team members and laboratory visitors is essential. Fluency in English and good computer skills are mandatory Postdoctoral Fellowship in Molecular Biology Location: Grenoble, France Staff Category: Postdoctoral Fellow Contract Duration: up to 18 months Grading: N/A Closing Date: 31.October 2012 Reference number: GR_00045 Job Description The European Molecular Biology Laboratory (EMBL) is one of the highest ranked scientific research organizations in the world. The Headquarters Laboratory is located in Heidelberg (Germany) and the outstations are in Grenoble (France), Hamburg (Germany), Hinxton (UK) and Monterotondo (Italy). EMBL is seeking to recruit a postdoctoral researcher to join the team of Darren Hart. The position is funded by the European Framework 7 BioStruct-X project and is a part of a collaboration to develop a new expression technology for structural biology that can be applied to challenging human proteins. The project will combine random library technologies and high throughput robotics developed at EMBL (PubMed ID: 20206698, 21515383 & 21364980) with parallelised mammalian expression approaches from the Aricescu lab, University of Oxford (PMID: 17001101). The subjects of the method development will be medically important human proteins where the aim is to initiate structural and functional studies. Qualifications and Experience The successful applicant should hold a Ph.D. in molecular biology and have experience of transfecting and handling mammalian cell lines, as well as being a skilled molecular biologist. He/she should be able to work independently and take responsibility for his/her own project. Strong teamwork and communication skills are required as well as reliability, attention to detail and effective time management. Motivation to work in a multidisciplinary and international environment is fundamental to this position. Good communication and presentation skills and fluency in English are expected. Application Instructions Please apply online through www.embl.org/jobs Please note that appointments on fixed term contracts can be renewed, depending on circumstances at the time of the review. -- ********************************************************************** Dr. Darren Hart, Team Leader High Throughput Protein Lab Grenoble Outstation European Molecular Biology Laboratory (EMBL) ********************************************************************** www.embl.fr/research/unit/hart/index.html For funded access to ESPRIT construct screening via EU FP7 PCUBE: http://tinyurl.com/ydnrwg4 **********************************************************************
