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From: CCP4 bulletin board on behalf of Eleanor Dodson
<176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk>
Sent: Saturday, April 21, 2018 1:36 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Rfree going up after refinement
I really need more i
I really need more information to comment properly.
However just check that you are using the same reflections for both Rfree
calculations.
An Rfree of 26% seems low to me from a Se met phased structure at 2.1A -
there are usually small corrections to be made, What is the Rwork?
Maybe attach the
Dear all
I have got my se-phase structure with ccp4-CRANK2-SAD, and its Rfree is 0.26
when CRANK2 output, but the Rfree is higher to 0.36 when I refine it with
refmac or phenix-refinement. does it mean I got a wrong phase, or I didn't got
the right parameters when refinement.BTW, the resolution
maybe those loops are right - ish.
Eleanor
kumar wrote:
Dear members,
I am refining a structure and I notice that when I delete loops that have
none, or very little density, the Rfree (which is around 40% at 3.4A) goes
up instead of going down. Much of the remainder of the molecule is in good
d
Dear members,
I am refining a structure and I notice that when I delete loops that have
none, or very little density, the Rfree (which is around 40% at 3.4A) goes
up instead of going down. Much of the remainder of the molecule is in good
density. Wondering how it works?
Thank you.
Kumar
