Re: [ccp4bb] Rhodamine in FPLC

2017-06-10 Thread Artem Evdokimov
30% acetic acid solution should wash it out (exercise some care, it's quite a strong vinegar). Hypochlorite and peroxide will bleach the stain, but technically speaking the leftover oxidized species will still be stuck in there, just colorless. Artem www.harkerbio.com "making lots of *really tin

Re: [ccp4bb] Rhodamine in FPLC

2017-06-09 Thread Tomas Malinauskas
Dear Reza, I used hydrogen peroxide (~0.1-2%?) to remove rhodamine stuck on Superdex columns. "Pink" columns turned "white" after this kind of treatment. Hope that helps, Tomas On Fri, Jun 9, 2017 at 10:43 PM, Reza Khayat wrote: > Hi, > > Sorry for the non crystallography question. We're using Rh

[ccp4bb] Rhodamine in FPLC

2017-06-09 Thread Reza Khayat
Hi, Sorry for the non crystallography question. We're using Rhodamine for labeling some material that we need to run through our FPLC. It seems that the Rhodamine is getting stuck to the PEEK tubing. Anyone has experience with successfully removing the Rhodamine from the tubing? Thanks. Best w