[ccp4bb] SUMARY: Optimization of needle crystals

2008-04-17 Thread Ngo Duc Tri
Dear ccp4 Users, I would like to say thank you to all of your valuable advices to optimize the needle crystal and related problems when dealing with membrane protein. Here I report the summary of your advices to help other students who got the same problem: 1. Additive screening from Hampton

Re: [ccp4bb] SUMARY: Optimization of needle crystals

2008-04-17 Thread Christophe Wirth
Hi, I can confirm what Bert Van Den Berg wrote about the round shaped crystals. It seems to appear often with DDM (in my case too, even sometimes in several drops per screen) and first looks like phase separation. Then it starts to be hard and is looking like a small CD under the microscope.

Re: [ccp4bb] SUMARY: Optimization of needle crystals

2008-04-17 Thread Van Den Berg, Bert
); 508 856 1211 (lab) e-mail: [EMAIL PROTECTED] http://www.umassmed.edu/pmm/faculty/vandenberg.cfm -Original Message- From: CCP4 bulletin board on behalf of Ngo Duc Tri Sent: Thu 4/17/2008 4:09 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] SUMARY: Optimization of needle crystals Dear ccp4