Re: [ccp4bb] Self rotation function calculation

2008-11-25 Thread Karsten Schaefer
Hi Sympath, Did you try to change the default Search radius? To my understanding MOLREP estimats the search radius somehow from the volume of the asymmetric unit and not from the expected radius of the monomer (you don't give the program the number of expected molecules). The signal of the SRF

Re: [ccp4bb] Self rotation function calculation

2008-11-25 Thread Sampath Natarajan
Dear All, Thank you very much for the reply and suggesions regarding my self rotation function calculation. Really I appreciate who are all responded me regarding my query. Now I got some idea about my structure in the assymetric unit. Thanking you once again. Sincerely yours, Sampath On Thu,

Re: [ccp4bb] Self rotation function calculation

2008-11-21 Thread Claudine MAYER
Hi Sampath, We had a very similar case, an octamer (422 symmetry) in C2 space group. What saved us for solving the structure (and by the way interpreting the self-rotation function !!) was a low resolution electron microscopy envelope (silver stain and cryo-EM) that gave us the organisation of