Re: [ccp4bb] Self rotation function interpretation

2013-02-11 Thread R. M. Garavito
Emma, You need to provide more information. Luckily, I222 is orthorhombic, so a* is along a, b* is along b, and c* is along c. This makes things so much easier to interpret. I hope the plotting conventions you used are typical: b along +y (North pole), a along +x (to the right), and c perpen

Re: [ccp4bb] Self rotation function interpretation

2013-02-10 Thread Fulvio Saccoccia
Did you perform Matthews analysis? It should give you some indications on the number of monomers in the asymmetric unit. Can you send the list of peaks? It should be a file with .tab extension. Cheers Fulvio Saccoccia Dept. of Biochemical Sciences Sapienza University of Rome Il 10/02/2013 05: