Re: [ccp4bb] Sigma Cutoff in HKL2000 Data Processing

2009-10-23 Thread Jose Antonio Cuesta Seijo
I guess you mean higher redundancy/completeness in the HIGHER resolution shells. Rather than decreasing that sigma cutoff, a better solution is to increase the profile fitting radius. The default value is fine for most lab-based detectors, but too small for most (larger) synchrotron detectors.

[ccp4bb] Sigma Cutoff in HKL2000 Data Processing

2009-10-22 Thread protein.chemist protein.chemist
What is the Sigma Cutoff that one should use for Data Processing using HKL2000. Is there a minimum or maximum value. Also if there is a variation then what all does it depend on. Thanks in Advance for your help. Mariah -- Mariah Jones Department of Biochemistry University of Florida

Re: [ccp4bb] Sigma Cutoff in HKL2000 Data Processing

2009-10-22 Thread William G. Scott
Dear Mariah: On Oct 22, 2009, at 7:18 AM, protein.chemist protein.chemist wrote: What is the Sigma Cutoff that one should use for Data Processing using HKL2000. None. Is there a minimum or maximum value. There are those who suggest negative intensities require a good imagination when

Re: [ccp4bb] Sigma Cutoff in HKL2000 Data Processing

2009-10-22 Thread Ethan Merritt
On Thursday 22 October 2009, protein.chemist protein.chemist wrote: What is the Sigma Cutoff that one should use for Data Processing using HKL2000. You need to narrow your question. Which sigma cutoff are you talking about? Do you mean the one on the Index panel? It controls the automatic

Re: [ccp4bb] Sigma Cutoff in HKL2000 Data Processing

2009-10-22 Thread Edward A. Berry
The default sigma cutoff (in denzo) is -3 and I wouldn't override that without good reason. The cut-off is applied before averaging equivalent reflections (but after adding partials?). If you reject all negative observations of a particular reflection and average the remaining positive