Hello,
Can anyone let me know how to calculate solubilisation and refolding
efficiency. We perform solubilisation and then refolding and check by HPLC
if refolding is completed or not [single peak on HPLC]. how do i determine %
efficiency of refolding. kindly guide me.
regards,
megha
How:
1. measure total protein before refolding (eg by absorbance at 280)
2. Purify refolded fraction by gel filtration in FPLC
3. measure total protein after refolding
4. calculate efficiency ...
Why?
I don't see the reason to know the efficiency. If after refolding I
would get enough in a
